38 research outputs found
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Applications in Low-Power Phased Array Weather Radars
Low-cost X-band radars are an emerging technology that offer significant advantages over traditional systems for weather remote sensing applications. X-band radars provide enhanced angular resolution at a fraction of the aperture size compared to larger, lower frequency systems. Because of their low cost and small form factor, these radars can now be integrated into more research and commercial applications. This work presents research and development activities using a low-cost, X-band (9410 MHz) Phase-Tilt Radar. The phase-tilt design is a novel phased array architecture that allows for rapid electronic scanning in azimuth and mechanical tilting in elevation, as a compromise between cost and performance.
This work focuses on field studies and experiments in three meteorological applications. The first stage of research focuses on the real-world application of phased array radars in forest fire monitoring and observation. From April to May 2013, a phase-tilt radar was deployed to South Australia and underwent a field campaign to make polarimetric observations of prescribed burns within and around the Adelaide Hills region. Measurements show the real-time evolution of the smoke plume dynamics at a spatial and temporal resolution that has never before been observed with an X-band radar. This dissertation will perform data analysis on results from this field campaign. Results are compared against existing work, theories, and approaches.
In the second stage of research, field experiments are performed to assess the data quality of X-band phased array radars. Specifically, this research focuses on the measurement of and techniques to improve the variance of weather product estimators for dual-polarized systems. Variability in the radar products is a complicated relationship between the radar system specifications, scanning strategy, and the physics governing precipitation. Here, the variance of the radar product estimators is measured using standard radar scanning strategies employed in traditional mechanical antenna systems. Results are compared against adaptive scan strategies such as beam multiplexing and frequency diversity. This work investigates the improvement that complex scanning strategies offer in dual-polarized, X-band phased array radar systems.
In the third stage of research, simulations and field experiments are conducted to investigate the performance benefits of adaptive scanning to optimize the data quality of radar returns. This research focuses on the development and implementation of a waveform agile and adaptive scanning strategy to improve the quality of weather product estimators. Active phased array radars allow radar systems to quickly vary both scan pointing angles and waveform parameters in response to real-time observations of the atmosphere. As an evolution of the previous research effort, this work develops techniques to adaptively change the scan pointing angles, transmit and matched filter waveform parameters to achieve a desired level of data quality. Strategies and techniques are developed to minimize the error between observed and desired data quality measures. Simulation and field experiments are performed to assess the quality of the developed strategies
Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field